MCOLN1 Knockout cell line (T98G)

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location： Home > Products > KO Cell Lines > MCOLN1 Knockout cell line (T98G)

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MCOLN1 Knockout cell line (T98G) Order

MCOLN1 Knockout cell line (T98G)
Catalog#
YKO-H792
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MCOLN1 Knockout cell line (T98G)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MCOLN1 Knockout cell line（T98G）
Cell Morphology
Fibroblast，adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
MCOLN1
Gene ID
57192
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate 100 mM Solution
Accession
CVCL_E1CJ(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:T98G		 STR Info (Cell bank)
Cell Line:T98G [T98-G]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10121012
D13S317 13131313
D7S820 910910
D16S539 13131313
VWA 17201720
TH01 79.379.3
AMEL XYXY
TPOX 8888
CSF1PO 10121012
D12S391 1718
FGA 2122
D2S1338 1924
D21S11 2832.2
D18S51 1316
D8S1179 1314
D3S1358 1616
D6S1043 1414
PENTAE 1616
D19S433 1213
PENTAD 1011
D1S1656 1417
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCOLN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the T98G cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCOLN1 gene knockout MCOLN1 Knockout cell line（T98G） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MCOLN1 Knockout cell line (T98G)
MCOLN1 Knockout cell line (T98G)
Catalog#
YKO-H792
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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